名稱

NCI-H146 [H146]（人小細胞肺癌細胞） (STR鑒定正確)

別稱

H146; H-146; NCIH146

種屬

人類

年齡（性別）

男性，59歲

組織來源

肺

生長特性

懸浮細胞

細胞形態(tài)

淋巴母細胞樣

背景描述

Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected.

生長培養(yǎng)基

RPMI-1640＋10% FBS＋1% P/S

推薦傳代比例

1:2-1:3

推薦換液頻率

2~3次/周

凍存條件

凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度：液氮

培養(yǎng)條件

氣相：空氣，95%；CO2，5%溫度：37℃

基因表達情況

vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -

注意事項

該細胞為懸浮細胞，請注意離心收集細胞懸液；請勿直接倒掉細胞培養(yǎng)液。

保藏機構(gòu)

ATCC; HTB-173